This year, our worldwide
chromatography community will be celebrating 50 year anniversary of high performance
liquid chromatography. 13 August 1966 Csaba Horvath and Seymour Lipsky
are published in Nature their report about first high pressure separation
of organic compounds on a self-packed ion exchange chromatographic column,
entitled. “Use of Liquid Ion Exchange Chromatography for the Separation of
Organic Compounds”.
Year latter was introduced first
commercial HPLC instrument by Waters. To emphasize the major technological
difference from typical at that time low pressure applications, HPLC meaning
was “High Pressure Liquid Chromatography”.
Before 1966 liquid chromatography
utilized either gravity flow or low pressure pumps for an eluent delivery.
Typical applications there are protein separations using ion exchange and gel
filtration, inorganic anion exchange chromatography and normal phase
separations of various organic substances using self – packed silica columns.
Isolation range was varied from analytical to preparative.
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