The accurate
measurement of steroid hormones is the fulcrum of modern endocrinology. Our aim
was to develop a mass spectrometry coupled
with a liquid chromatographic method for 17-hydroxyprogesterone,
androstenedione, 11-desoxycortisol, 21-desoxycortisol and cortisol serum
quantification simultaneously.
The samples were
submitted to isotopic internal standards addition, protein precipitation and
two dimensional liquid chromatography consists of trapping column and
reverse-phase C18 analytical column.
For all compounds
tested functional sensitivity was less than 0.5 ng/mL, precision was less than
15%, recovery ranged from 93% to 120%, linearity ranged from 89% to 111%, and
accuracy was considered adequate.
No comments:
Post a Comment