Development and Application of Liquid Chromatography in Life Sciences

Liquid chromatographic approaches cover a wide range of applications today. It is closely relevant in scientific studies, which provides a powerful identification of certain components that will be effective for curing specificdiseases.

Liquid Chromatography

Especially important, LC methods can assist us for isolation and purification of therapeutic drugs that benefit for the development of medical industry. As the development of liquid chromatography, the single effective component within a drug can be separated and purified under extreme purity requirements from a biological system with great complexity and abundance. However, improvement of LC methods is still urgently needed for better application of this method to benefit our lives.

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Analytical Performance of LC-MS/MS Method for Simultaneous Determination of Five Steroids in Serum

The accurate measurement of steroid hormones is the fulcrum of modern endocrinology. Our aim was to develop a mass spectrometry coupled with a liquid chromatographic method for 17-hydroxyprogesterone, androstenedione, 11-desoxycortisol, 21-desoxycortisol and cortisol serum quantification simultaneously.

The samples were submitted to isotopic internal standards addition, protein precipitation and two dimensional liquid chromatography consists of trapping column and reverse-phase C18 analytical column.

For all compounds tested functional sensitivity was less than 0.5 ng/mL, precision was less than 15%, recovery ranged from 93% to 120%, linearity ranged from 89% to 111%, and accuracy was considered adequate.

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