The aim of this
study was to create universal chimeric conjugates able to react with both avian
and mammalian immunoglobulins to be used as a reagent
in Enzyme-linked Immunosorbent Assays (ELISAs).
The periodate
method was used in the conjugation process of linking horseradish
peroxidase to immunoglobulin-binding bacterial proteins. ELISAs were used
to prove the efficacy of the conjugates, namely newly synthesize conjugates
(NSC) in the detection of immunoglobulins.
All NSC bound to
mammalian immunoglobulins, but failed to bind avian immunoglobulin Y (IgY),
with the exception of the SpLAG-anti-IgY-HRP that was the most versatile
binding to the all panel of purified immunoglobulin and sera.
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