This year, our worldwide chromatography community will be
celebrating 50 year anniversary of high performance liquid chromatography. 13
August 1966 Csaba Horvath and Seymour Lipsky are published in Nature their
report about first high pressure separation of organic compounds on a self-packed
ion exchange chromatographic column, entitled: “Use of Liquid Ion Exchange Chromatography for
the Separation of Organic Compounds”.
Year latter was introduced first commercial HPLC instrument
by Waters. To emphasize the major technological difference from typical at that
time low pressure applications, HPLC meaning was “High Pressure Liquid
Chromatography”.
Before 1966 liquid chromatography utilized either gravity
flow or low pressure pumps for an eluent delivery. Typical applications there
are protein separations using ion exchange and gel filtration, inorganic anion
exchange chromatography and normal phase separations of various organic
substances using self – packed silica columns.
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