Thursday, 25 August 2016

Investigation of Isotopic Abundance Ratio of Biofield Treated Phenol Derivatives Using Gas Chromatography-Mass Spectrometry

Butylatedhydroxytoluene (BHT) is a crystalline stable solid, but it is light-sensitive and reactive to acid chlorides, acid anhydrides, and oxidizing agents. BHT is used as anantioxidant in many products including food, pharmaceuticals, rubbers, paint, and petroleum products. Its antioxidant mechanism was well studied and divided into two steps. In the first step, it forms a stable phenoxyl radical, which further forms the parent and a quinone methide (QM). QM is a reactive electrophilic species that easily forms adducts with nucleophiles or polymerize in the next step. 

Chromatography-Mass Spectrometry
Adduct formation between the nucleophilic groups of the active pharmaceutical ingredients with QM in pharmaceutical formulations is very much possible. In food preservation, BHT reacts with atmospheric oxygen preferentially rather than oxidizing food materials, thereby protecting them from decomposition. It is used to preserve food odor, color, and flavor.. Many polymerization inhibitors (e.g., phenols) work best in the presence of oxygen because they intercept peroxyl radicals and decelerate oxygen consumption while stopping chain propagation. 4-MP is an inhibitor of this type. 4-MP has tremendous ability to quench peroxyl radicals and alkyl radicals via hydrogen abstraction mechanism, which leads to the formation of a phenoxyl radical . 

The phenoxyl radical is less reactive because it is stabilized by the resonance effect. The antimicrobial activity of phenolic compounds is highly dependent upon the chemical structure of the molecules. The bactericidal actions of substituted phenols and the normal alkyl derivatives of p-chlorophenols were examined against Gram-negative and Gram-positive bacteria. The antibacterial potential of essential oils of sweet basil Ocimum basilicum L. (Lamiaceae) containing 1.8% 4-MP and methanol extracts was evaluated for controlling the growth range of food-borne pathogenic bacteria. It was reported that BHT at high levels as well as at lower levels found in foods might have anti-cancer properties, possibly by damaging free radicals or by stimulating the production of enzymes that detoxify carcinogens. On the other hand 4-MP is carcinogenic to the fore-stomach.

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