Monday, 19 June 2017

Qualitative and Quantitative Analysis of Dan-Deng-Tong-Nao Capsules by HPLC-DAD-MS/MS

Quantitative Analysis of Dan-Deng-Tong-Nao
Dan-Deng-Tong-Nao capsules (DDTNCs), a traditional Chinese medicinal preparation consisting of Radix Salvia miltiorrhiza, Radix Pueraria lobata, Erigeron breviscapus, Rhizoma chuanxiong has been used for the treatment of apoplexy and the syndrome of apoplexy involving both collaterals and meridians caused by obstruction of collaterals by blood stasis in China.

In this paper, a HPLC-DAD-MS/MS method was successfully developed for qualitative and quantitative analysis of major components in DDTNCs for the first time.

Twenty-six compounds were identified by comparison of their retention times and MS spectra with those elucidated standards or recorded literatures, and six of them (danshensu, 5-hydroxymethylfurfural, 3,4-dihydroxybenzaldehyde, epicatechin, puerarin and salvianolic acid A) were simultaneously determined by HPLC-DAD quantitatively.

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