Dan-Deng-Tong-Nao capsules (DDTNCs), a
traditional Chinese medicinal preparation consisting of Radix Salvia
miltiorrhiza, Radix Pueraria lobata, Erigeron breviscapus, Rhizoma chuanxiong
has been used for the treatment of apoplexy and the syndrome of apoplexy
involving both collaterals and meridians caused by obstruction of collaterals
by blood stasis in China.
In this paper, a HPLC-DAD-MS/MS method was
successfully developed for qualitative and quantitative analysis of major
components in DDTNCs for the first time. Twenty-six compounds were identified
by comparison of their retention times and MS spectra with those elucidated
standards or recorded literatures, and six of them (danshensu,
5-hydroxymethylfurfural, 3,4-dihydroxybenzaldehyde, epicatechin, puerarin and
salvianolic acid A) were simultaneously determined by HPLC-DAD quantitatively.
The analytical method was fully validated and
successfully applied for quantitative and fingerprint analysis of DDTNCs from
fifteen different production batches, results indicated that the proposed
approach was applicable for the routine analysis and quality control of DDTNCs
No comments:
Post a Comment