In the present
study, we introduced a novel, reliable, sensitive and highly precise capillary
zone electrophoresis (CZE) method for simultaneous separation and determination
of nalbuphine hydrochloride (NLB) and its related antagonist compounds naloxone
hydrochloride (NLX) and naltrexone hydrochloride (NLT) in bulk drug,
pharmaceuticals and human biological fluids.
The separation
conditions using CZE were optimized and the separation process was carried out using
fused silica capillary of total and effective lengths 57 and 50 cm,
respectively. The applied running buffer was acetate buffer 20 mmol L-1 of
pH=3.8 at a potential 15 kV.
The applied
internal solution (IS) was phenylethylamine (PEA). The developed method
displayed an excellent separation of the investigated drugs with linear
concentration ranges of 5-200, 20-240 and 10-280 μg mL-1 for NLB, NLX and NLT,
respectively.
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