White matter injury and degeneration are
common features of developmental and aging-associated diseases, yet their
pathobiological bases are poorly understood. However, recent advances in
Matrix-Assisted Laser Desorption Ionization instruments and chemistry have
provided critical tools for myelin-lipid analytical research.
This study characterizes Cigarette Smoke exposure
effects on frontal lobe lipid ion profiles in adult male A/J mice that had
been exposed to air for 8 weeks (A8), CS for 4 (CS4) or 8 weeks , or CS8
followed by 2 weeks recovery. MALDI data acquired by analysis of lipid extracts
plated onto a ground steel target (high through-put) were compared with Imaging
Mass Spectrometry.
MALDI-time-of-flight detected 120 lipid ions
with m/z’s of 600 to 1300 (phospholipids and sulfatides) in samples plated onto
the steel target or analyzed by IMS, but just 25 ions (18%) were detected by
both methods.
