Validation and Development of HPTLC Method for Simultaneous Estimation of Apigenin and Luteolin in Selected Marketed Ayurvedic Formulations of ‘Dashmula’ and in Ethyl Acetate Extract of Premna integrifolia L.

Dashmool (dashmul, dashamula) are specific combination of ten roots, famous Ayurvedic remedy mainly for strengthening body, promotes healthy elimination of toxins, tonic for kidney, liver, tonic in infertility, uterine tonic, used for anorexia, edema, anaemia, potent antioxidant and in disorders of nerves, bones, joints and muscles.

It contains ten roots in equal propotion under category as ‘Brihat Panchmul includes; Aegle marmelos, Premna integrifolia, Oroxylum indicum, Stereospermum suveolens, Gmelia arborea While Laghu panchmul includes; Solanum indicum, Solanum xanthocarpum, Uraria picta, Desmodium gangeticum and Tribulus terrestris.

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Validation and Development of HPTLC Method for Simultaneous Estimation of Apigenin and Luteolin in Selected Marketed Ayurvedic Formulations of ‘Dashmula’

Dashmula are specific ayurvedic combination of ten roots used for various disorders of liver, kidney, uterus. Standardisation of herbals are necessity for efficacy and quality parameter as per WHO guidelines.

Therefore, through this original research attempt was made to standardise one of ‘Dashmula’, P. integrifolia and selected batches of marketed formulation using Apigenin and Luteolin as active biological marker for simultaneous quantification and fingerprinting through developed and validated HPTLC techniques.

Developed mobile phase Toluene: Ethyl acetate: Formic acid (6:4:0.15) gave Rf (Retention factor) 0.39 and 0.29 for Standard Apigenin and Luteolin respectively at 347 nm iso absorptive wavelength.

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