Determination of
a single “fingerprint” mass spectrum of a biologically active compound, required
for compound identification in mixtures and biological
materials in such applied tasks as ecological monitoring and biological
imaging, may become a nontrivial problem for labile compounds whose mass
spectra depend strongly on the applied experimental conditions.
In the present
communication, qualitatively different mass spectral patterns obtained for the
bisquaternary ammonium salt ethonium Cat2+•2Cl– under varied conditions of
electrospray ionization and matrixassisted laser desorption/ionization (MALDI)
are described and systematized.
It is shown that
qualitative changes in the electrospray mass spectra of ethonium occur upon a
cone voltage increase from 10 V to 100 V, which are caused by the subsequent
appearance and destruction of several primary ions: the intact dication Cat2+, the
dication-counterion cluster Cat2+•Cl– and[Cat ‑ H]+ and [Cat ‑ CH3]+ ions.
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